This project addresses critical technical bottlenecks in current integrated sewage treatment systems, such as low nitrogen removal efficiency, unstable effluent quality, limited biofilm biomass enrichment, and high operational costs. It focuses on developing an innovative integrated anaerobic-aerobic reactor structure. By correlating the abundance of specific microbial communities with nitrite nitrogen (NO??-N) accumulation, the project aims to achieve precise quantification and control of partial nitrification and denitrification processes. It will further investigate the impacts of operational parameters, substrate factors, and environmental conditions on anaerobic ammonium oxidation (ANAMMOX)-based nitrogen removal, revealing synergistic mechanisms among influencing factors, microbial traits, and pollutant transformation. The ultimate goal is to create an integrated, intelligent, and highly efficient decentralized wastewater treatment system tailored for rural areas. This will establish a distinctive product line under the Haitian brand, contributing to improved rural water quality and supporting national rural revitalization efforts.